| ncRNA name | B-RafV599E siRNA |
| ncRNA Category | siRNA |
| Disease name | uveal melanoma (UM) |
| Species | Homo sapiens |
| Tissues/Cell_line | choroidal melanoma cell line OCM-1, MKT-BR and SP-6.5, normal choroidal melanocytes |
| Methods | siRNA transfection, Western blot, immunoblot, PCR, transformation assay |
| Expression pattern | associated |
| Functional description | Three melanoma cell lines (OCM-1, MKT-BR, and SP-6.5) showed a 10-fold increase in endogenous B-RafV599E kinase activity and a constitutive activation of the MEK/ERK pathway that is independent of Ras. This, as well as melanoma cell proliferation, was strongly diminished by siRNA-mediated depletion of the mutant B-Raf protein. |
| PubMed ID | 12917419 |
| Year | 2003 |
| Title | Mutation of B-Raf in human choroidal melanoma cells mediates cell proliferation and transformation through the MEK/ERK pathway. |
| Drug-related ncRNA | NO |
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